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Analysis
of blood for specific and total IgE
The
laboratory team received and maintained a serum bank from ECRHS centres
where they carried out assays for serum total IgE and specific IgE for
D. pteronyssinus (d1), cat (e1), timothy grass (g6) and Cladosporium (m2)
using the Pharmacia CAP System and standard operating procedures. A study
database of serum IgE assay results was created for central analyses.
Quality control schemes, as organised by Pharmacia and Upjohn Diagnostics
and in-house, were established to ensure compatibility with methods used
in the first survey.
Serum
samples were prepared for specific IgE tests
using the Pharmacia CAP System positioning guide
Analysis
of dust
The laboratory staff· carried out house dust mite allergen assay
for Der p 1 and Der f 1 and Der 2 and maintained the study database of
house dust mite allergen assay results for central analyses. A quality
control programme was set up consisting of solutions of known concentrations
of Der p 1, Der f 1 and Der 2 included in each assay.
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Mattress
dust samples were sieved prior to extraction
and assay for house dust mite allergens
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Microtitre
plates were set up for enzyme-linked
immunoassay for house dust mite allergens
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Dust
samples were incubated with monoclonal antibodies
specific for 3 different house dust mite allergens
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